Real-time cellular analysis (RTCA) (ACEA Biosciences, USA) was used to evaluate cell proliferation [16 (link)]. First, the baseline value was measured in 100 μl of culture medium preincubated at 37 °C in a cell incubator for 1 h. Then, the cells were seeded in wells at a density of 2000 cells per well. The attachment and proliferation of cells were measured by the RTCA system for 6 and 168 h, respectively.
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