Inhibition of HIF-1α in NSCLC Cells

Human NSCLC cell lines A549, H1299, human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC). Cells were cultured in the complete RPMI1640 medium containing 10% inactivated newborn bovine serum (FBS, HyClone, Logan, UT, USA) and 1% penicillin/streptomycin. They were cultured at 37° C in an incubator with saturated humidity and 5% CO₂. The medium was changed every 2 to 3 days. Cells were trypsinized and sub-cultured with 0.25% trypsin during the logarithmic growth phase. To inhibit HIF-1α in A549 cells, SKPV-3 cells were treated with PX-478 (40 μM) (Selleck Chemicals, Houston, TX, USA) for 18 hours [55 (link)].