Characterizing TCRα Repertoire in B6 Mice

Wild-type (WT) C57BL/6 (B6) mice were purchased from Charles River Laboratories; B6.CD45.1 mice were from The Jackson Laboratory; the new Traj18^−/− mice expressing undisturbed TCRα chain repertoire, except for Jα18, on B6 background were described (20 (link)). Mice were maintained in the animal facility of RIKEN IMS under specific pathogen-free conditions and were used at 8–10 weeks of age. All animal experiments were approved by RIKEN Animal Care and Use Committee.

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Dashtsoodol N., Shigeura T., Tashiro T., Aihara M., Chikanishi T., Okada H., Hanada K., Sano H., Kurogi A, & Taniguchi M. (2017). Natural Killer T Cell-Targeted Immunotherapy Mediating Long-term Memory Responses and Strong Antitumor Activity. Frontiers in Immunology, 8, 1206.

Publication 2017

) C57BL/6 (B6) mice B6.CD45.1 mice

Animal Mice River Specific pathogen free

Corresponding Organization : RIKEN Center for Integrative Medical Sciences

Other organizations : Mongolian National University of Medical Sciences, Niigata University of Pharmacy and Applied Life Sciences

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Variable analysis

independent variables

Mouse strains (WT C57BL/6, B6.CD45.1, Traj18-/- on B6 background)

dependent variables

Not explicitly mentioned

control variables

Age of mice (8-10 weeks)
Housing conditions (specific pathogen-free)

controls

Positive control: WT C57BL/6 (B6) mice
Negative control: Not explicitly mentioned

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