Protocol detail

miRNA Mimics and Knockdown Protocol

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The miRNA mimics and 5′- or 3′- biotin linked miRNA mimics were synthesized by RiboBio Co., Ltd. (Guangzhou, China). A short interfering RNA (siCDH1) was used to knockout E-cadherin expression and a dsControl which lacks of significant homology to all known human sequences was synthesized and used as a negative control [12 (link), 44 (link)]. Besides, Lenti-miR-373 and Lenti-dsControl were structured by GenePharma (Shanghai, China). The sequences of the custom dsRNAs and miRNA are listed in Supplementary Table 1.

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Zhang Q., Wang C., Miao S., Li C., Chen Z, & Li F. (2017). Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth and metastasis. Oncotarget, 8(55), 93969-93983.

Publication 2017

miRNA mimics Lenti-dsControl

Biotin Cadherin Dsrnas Human Mirna Short interfering rna

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Variable analysis

independent variables

MiRNA mimics and 5′- or 3′- biotin linked miRNA mimics
Short interfering RNA (siCDH1) used to knock out E-cadherin expression
DsControl which lacks significant homology to all known human sequences
Lenti-miR-373
Lenti-dsControl

dependent variables

Measured outcomes (not explicitly specified)

control variables

DsControl which lacks significant homology to all known human sequences
Lenti-dsControl

controls

DsControl which lacks significant homology to all known human sequences
Lenti-dsControl

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