Trophoblast Cell Inflammation Regulation by lncRNA BC030099

Human trophoblast cell HTR-8/SVneo was purchased from Tongpai (Shanghai, China) Biotechnology Co., Ltd., and culture conditions were as follows: RPMI-1640 medium and 10% fetal bovine serum (FBS). LPS was applied to induce cellular inflammation [15 (link)], and in addition, lncRNA BC030099 was knocked down, which are grouped into the si-control, si-con+LPS, si-BC030099, and si-BC030099+LPS groups. In addition, we added 10 μM NF-κB-specific inhibitor JSH-23 (Merck, Germany) [16 (link)], grouped as the control, LPS, JSH-23, and LPS+JSH-23 groups. lncRNA BC030099 expression was knocked down. si-BC030099 and negative control si-control were synthesized by Sangon Biotechnology Co., Ltd. (Shanghai, China).