Briefly, 5 × 104–2 × 105/mL H9c2 cells were planted on a 96-well plate and incubated at 37°C for 6 hours, followed by adding the LDH solution (Mlbio, Shanghai, China) and incubating for another 1 hour. Lastly, the microplate reader (Mindray, Shenzhen, China) was used to measure the absorbance at 492 nm to determine the release of LDH [16 (link)].
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