Quantification of D2 receptor isoforms

Two isoforms of the D2R, D2L and D2S have been analyzed previously and a few modifications were observed [33 (link),34 (link)]. A detailed description of this analysis was reported in our previous study [26 (link)]. Glutaraldehyde-treated membranes were incubated in 5% skimmed milk overnight at 4 °C with rabbit polyclonal antibodies against D2L (1:2000) and D2S (1:5000) (AbClon, Inc., Seoul, Korea). After the membranes were washed three times, the primary antibodies were detected using peroxidase-labeled goat anti-rabbit IgG (H + L) (1:3000 for D2L and 1:5000 for D2S; Vector Laboratories) for 2 h at room temperature (25 °C).