MM cell lines (MM.1S, MM.1R, RPMI8226, INA6, Dox40, LR5, ARP1) and peripheral blood mononuclear cells from normal healthy donors were cultured in RPMI-1640 medium containing 10% fetal bovine serum and antibiotics. ANBL6 cells were kindly provided by Dr Robert Orlowski (Houston, TX, USA).45 (link) Tumor cells, BMSCs and pDCs were cultured as described previously.28 (link),29 (link) Helsinki protocol was followed for obtaining informed consent from all patients. Bortezomib, dexamethasone, lenalidomide or pomalidomide were obtained from Selleck Chemicals LLC (Houston, TX, USA), and OPA was obtained from Calbiochem (San Diego, CA, USA). Recombinant DUBs were obtained from Creative BioMart (Rpn11/ PSMD14, Shirley, NY, USA), Boston Biochem (USP1, USP7, Cambridge, MA, USA) and LifeSensors Inc. (USP2, USP4, USP5, USP8, USP20, Uch37, Malvern, PA, USA).
Protocol detail
Deubiquitinase Inhibition in Multiple Myeloma
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Antibiotics
Bortezomib
Cell lines
Cells
Cultured medium
Dexamethasone
Donors
Fetal bovine serum
Lenalidomide
Patients
Pdcs
Peripheral blood mononuclear cells
Pomalidomide
Tumor
Usp1
Usp7
Usp8
Corresponding Organization :
Other organizations : Harvard University, Brigham and Women's Hospital, Dana-Farber Cancer Institute
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Variable analysis
independent variables
- Bortezomib
- Dexamethasone
- Lenalidomide
- Pomalidomide
- OPA (obtained from Calbiochem)
- Recombinant DUBs (Rpn11/PSMD14, USP1, USP7, USP2, USP4, USP5, USP8, USP20, Uch37)
- Tumor cell growth/proliferation
- BMSC and pDC responses
- RPMI-1640 medium containing 10% fetal bovine serum and antibiotics
- Cell lines (MM.1S, MM.1R, RPMI8226, INA6, Dox40, LR5, ARP1, ANBL6)
- Peripheral blood mononuclear cells from normal healthy donors
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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