Genetic Manipulation of Drosophila Development

Fly culcture and crosses were performed according to standard procedures. Drosophila stocks used in this study include: UAS-Cut (#36496; Bloomington stock center (BDSC)); FRT19A, cut^C145 (#36496; BDSC); UAS-Wg-HA (#5918; BDSC); UAS-Arm-S10 (#4782; BDSC); UAS-cut-RNAi (#33967; BDSC); UAS-br-RNAi (#27272; BDSC); esg-Gal4, tubP-Gal80^ts, UAS-GFP (Biteau et al., 2008 (link); Micchelli and Perrimon, 2006 (link)); wg-Gal4 (Alexandre et al., 2014 (link)); wg(KO; NRT–Wg) (Alexandre et al., 2014 (link)); UAS-TCF-ΔN (#4785; BDSC); wg-lacZ (#1672; BDSC); Rab3-GFP (#62541; BDSC); UAS-CD8-RFP (#27392, BDSC); cut-intron2-Gal4 (#49818, BDSC); NRE-GFP (#30727; BDSC); UAS-Sgg.S9A (#5255; BDSC); UAS-EcR-DN (#6872; BDSC); UAS-wg-RNAi (#33902; BDSC); UAS-Br-Z1 (#51190; BDSC); UAS-Br-Z3 (#51192; BDSC); UAS-Br-Z4 (#51193; BDSC); FRT19A, dsh³ (#6331; BDSC); UAS-TCF (#4838; BDSC); UAS-Nipped-B-RNAi (#32406; BDSC); UAS-SA-RNAi (#33395; BDSC); UAS-Eip74EF-RNAi (#29353; BDSC); UAS-Eip75B (#26717; BDSC); UAS-white-RNAi (#33623; BDSC); Act5C-FRT>stop>FRT-lacZ.nls (#6355; BDSC).
wg-Gal4 transgenic flies were generated by integrating the Gal4 containing plasmid RIVGal4 (Baena-Lopez et al., 2013 (link)) into the attP site of wg flies (Alexandre et al., 2014 (link)).

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Xu K., Liu X., Wang Y., Wong C, & Song Y. (2018). Temporospatial induction of homeodomain gene cut dictates natural lineage reprogramming. eLife, 7, e33934.

Publication 2018

UAS-Arm-S10 UAS-br-RNAi wg-lacZ UAS-CD8-RFP NRE-GFP UAS-SA-RNAi

Drosophila Flies Lacz Plasmid Rnai Transgenic

Corresponding Organization :

Other organizations : Peking University, Center for Life Sciences

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Variable analysis

independent variables

Genetic manipulations (e.g., overexpression of Cut, Wg, Arm-S10, knockdown of Cut, br, Nipped-B, SA, Eip74EF)
Transgenic flies (e.g., UAS-Cut, FRT19A cut^C145, UAS-Wg-HA, UAS-Arm-S10, UAS-cut-RNAi, UAS-br-RNAi, esg-Gal4 tubP-Gal80^ts UAS-GFP, wg-Gal4, wg(KO; NRT–Wg), UAS-TCF-ΔN, wg-lacZ, Rab3-GFP, UAS-CD8-RFP, cut-intron2-Gal4, NRE-GFP, UAS-Sgg.S9A, UAS-EcR-DN, UAS-wg-RNAi, UAS-Br-Z1, UAS-Br-Z3, UAS-Br-Z4, FRT19A dsh^3, UAS-TCF, UAS-Nipped-B-RNAi, UAS-SA-RNAi, UAS-Eip74EF-RNAi, UAS-Eip75B, UAS-white-RNAi, Act5C-FRT>stop>FRT-lacZ.nls)

dependent variables

Outcomes related to the genetic manipulations, such as changes in gene expression, cellular phenotypes, and developmental processes (not explicitly stated)

control variables

Standard procedures for fly culture and crosses
Bloomington Drosophila Stock Center as the source for various Drosophila stocks

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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