Quantifying MRSA Biofilm Disruption

The construction of MRSA biofilm was described previously (Alalaiwe et al., 2018 (link)). After the CoQ₀ (78–312 µg/ml) or cetylpyridinium chloride (CPC, 75 µg/ml) treatment for 24 h, the biofilm was rinsed using PBS to remove the loosely adherent planktonic MRSA and was then suspended in PBS. The recovered MRSA outside the biofilm and the MRSA inside the biofilm were placed in an agar plate for 24 h to count CFU. The CoQ₀- or CPC-treated biofilm was stained using Live/Dead BacLight^®. The 3D structure, thickness, and fluorescence of the biofilm were observed by confocal microscopy (Leica).