The status of the outer mitochondrial membrane potential (Ψm) was assessed using the JC-1 mitochondrial potential assay kit from Cayman Chemical Company (Ann Arbor, MI, USA) (16 (link)). The cytofluorimetric cationic dye 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine iodide (JC-1) was used to assess the ΔΨm. The selective dye changes color from green to red as the Ψm increases. Cells were seeded in plate before being treated with Hap H (0.0016 µM) for 24 h. Thereafter, the cells were harvested using trypsin-EDTA from Gibco. Stain was added to the cells followed by incubation for 15 min at 37°C in 5% CO2. Analysis was performed using BD FACS Canto II flow cytometer. Fluorescence was assessed using excitation wavelength of 520–570 nm and emission wavelength 570–610 nm, respectively.