Animals were divided into the following experimental groups and were exposed to the same treatment protocol as in series 1:
TGR + vehicle (n = 8)
HanSD + vehicle (n = 7)
TGR + c-AUCB (n = 8)
HanSD + c-AUCB (n = 7)
Since it is now recognized that plasma and tissue ANG II concentrations in anesthetized animals are higher than those obtained from decapitated conscious rats and that normotensive animals exhibit greater increases in renin secretion in response to anesthesia and surgery than ANG II-dependent hypertensive intrarenal renin-depleted animals [22 (link)], in the present study rats from each experimental group were killed by decapitation and plasma and tissue collected. Plasma, whole kidney, and LV myocardial ANG II levels were measured by radioimmunoassay as described previously [22 (link)]. This approach for blood and tissue sampling and ANG II assay, which is routinely used in our laboratory, allows comparison of the present results with those of our previous studies evaluating the role of the renin-angiotensin system (RAS) in the pathophysiology of hypertension and tissue damage [14 (link),21 (link)-23 (link)].
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Corresponding Organization : Czech Academy of Sciences, Institute of Physiology
Other organizations :
Institute of Clinical and Experimental Medicine, University of Bonn, University of California, Davis, Medical College of Wisconsin, Charles University
Blood and tissue sampling method (decapitation of conscious rats)
ANG II assay method (radioimmunoassay)
controls
Positive control: None specified
Negative control: None specified
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