Protocol detail

Immunohistochemical Analysis of Cellular Proteins

Find Similar Protocols

All performed as reported³⁴, using the following reagents: the mouse monoclonal anti-PR (6A1; Cell Signaling, Beverly, MA, USA), anti-cytokeratin (C11; Santa Cruz), anti-αSMA (CGA7; Santa Cruz), anti-FAP (SS-13; Santa Cruz), anti MT-MMP-1 (SC-373908; Santa Cruz), anti-tubulin (Sigma-Aldrich), anti-FAK and anti P-Tyr397 FAK (both from BD Bioscience) antibodies; the rabbit polyclonal anti-AR (N-20; Santa Cruz), anti-ERα (HC-20; Santa Cruz), anti-vimentin (H-84; Santa Cruz), anti-FlnA (4762S; Cell Signaling) and anti-integrin β1 (Ab1952; Millipore) antibodies. The mouse monoclonal anti-AR (441; Santa Cruz) antibody was used to immune-precipitate and detect AR in Co-IP experiments. Rac pull-down assay was done^{20 (link)}, using the Rac activation kit (Upstate Biotechnology, Burlington, MA, USA). The ECL system (GE Healthcare) was used to reveal immune-reactive proteins.

Free full text: Click here

Di Donato M., Zamagni A., Galasso G., Di Zazzo E., Giovannelli P., Barone M.V., Zanoni M., Gunelli R., Costantini M., Auricchio F., Migliaccio A., Tesei A, & Castoria G. (2021). The androgen receptor/filamin A complex as a target in prostate cancer microenvironment. Cell Death & Disease, 12(1), 127.

Publication 2021

anti-tubulin anti-FAK anti-AR (N-20; anti-ERα (HC-20; anti-AR (441; ECL system

Antibodies Antibody Assay Cytokeratin Integrin Mouse Mt mmp 1 Proteins Rabbit Tubulin Vimentin αsma

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Mouse monoclonal anti-PR (6A1; Cell Signaling, Beverly, MA, USA)
Anti-cytokeratin (C11; Santa Cruz)
Anti-αSMA (CGA7; Santa Cruz)
Anti-FAP (SS-13; Santa Cruz)
Anti MT-MMP-1 (SC-373908; Santa Cruz)
Anti-tubulin (Sigma-Aldrich)
Anti-FAK and anti P-Tyr397 FAK (both from BD Bioscience) antibodies
Rabbit polyclonal anti-AR (N-20; Santa Cruz)
Anti-ERα (HC-20; Santa Cruz)
Anti-vimentin (H-84; Santa Cruz)
Anti-FlnA (4762S; Cell Signaling)
Anti-integrin β1 (Ab1952; Millipore) antibodies
Mouse monoclonal anti-AR (441; Santa Cruz) antibody was used to immune-precipitate and detect AR in Co-IP experiments
Rac pull-down assay was done, using the Rac activation kit (Upstate Biotechnology, Burlington, MA, USA)

dependent variables

No dependent variables are explicitly mentioned.

control variables

No control variables are explicitly mentioned.

controls

No positive or negative controls are explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!