The antibacterial effect of the clary sage extracts on P. aeruginosa (ATCC 27853) and methicillin-resistant Staphylococcus aureus (MRSA 4262) was screened in the laboratory of the Department of Medical Microbiology and Immunology (Medical School, University of Pécs, Hungary). For bioautographic assay, bacteria were grown in 100 mL Brain–Heart Infusion Broth (BHI) (Sigma Aldrich Ltd., Darmstadt, Germany) at 37 °C in a shaker incubator at a speed of 60 rpm for 12 h [53 ]. The bacterial suspension was diluted with fresh nutrient BHI to an OD600 of 0.4, which corresponds to approximately 4 × 107 colony-forming units (cfu)/ mL [16 (link)].
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