A glutaminase inhibitor, compound 968 (968; EMD Millipore, Billerica, MA), was prepared by dissolving 968 in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO) at 30 mM. 36 hours prior to ESV harvesting, cells were treated with 968 at 10 μM (71 (link), 72 (link)); a total of 1.67 μL of 968 in DMSO was added to 5 mL of culture medium (0.03% by volume). Following the protocol described in Section 2.3, cells were treated with 10 μM 968 under serum-starved conditions for 12 hours. Subsequent harvesting and centrifugation steps are identical to those previously described.
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Glutaminase Inhibitor for Extracellular Vesicle Harvesting
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Other organizations : Cornell University
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Variable analysis
independent variables
- Concentration of compound 968 (10 μM)
- Harvesting of extracellular vesicles (ESVs)
- Time of treatment with compound 968 (36 hours prior to ESV harvesting)
- Serum-starved conditions for 12 hours
- Preparation of compound 968 in DMSO at 30 mM
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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