Protein Analysis via Western Blot

Procedures for preparation of protein lysates and Western blots were followed as described [33] (link), [64] (link). For Western blot, 15 milligrams of whole-cell protein lysate was loaded per lane. Anti-V5 antibody (Pierce) was diluted 1∶5000 for use as the primary antibody. SWI2 antibody was obtained from Abcam (Ab3749). Protein purification prior to MS/MS analysis was performed using a slightly modified procedure [64] (link).

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Wang B., Kettenbach A.N., Gerber S.A., Loros J.J, & Dunlap J.C. (2014). Neurospora WC-1 Recruits SWI/SNF to Remodel frequency and Initiate a Circadian Cycle. PLoS Genetics, 10(9), e1004599.

Publication 2014

Ab3749

Anti antibody Antibody Cell Ms ms Protein Western blot Western blots

Corresponding Organization : Dartmouth College

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Variable analysis

independent variables

Protein purification procedure using a slightly modified procedure [64]

dependent variables

Protein levels measured by Western blot

control variables

Protein lysate preparation procedures followed as described [33], [64]
15 milligrams of whole-cell protein lysate loaded per lane
Anti-V5 antibody diluted 1:5000 used as the primary antibody
SWI2 antibody obtained from Abcam (Ab3749)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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