Generating Secondary Effector T Cells

Thy1.1⁺ OT-I cells (1.0 × 10⁴) were transferred i.v. and mice were infected with 10⁴ CFU Listeria monocytogenes strains expressing OVA APL epitope (LM-OVA APLs) (15 (link)) 24 h later. Secondary effectors were generated in 4 week post-infection mice by immunizing with 50 µg N4 OVA peptide (GenScript) emulsified in IFA (Gibco) in both hind foot pads. Five days later, draining popliteal LNs were collected and pooled for analysis. Serum was analyzed using Ready-Set-Go ELISA kit according to manufacturer’s instructions (eBiosciences).

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Krummey S.M., Chen C.W., Guasch S.A., Liu D., Wagener M., Larsen C.P, & Ford M.L. (2016). Enhanced Requirement for TNFR2 in Graft Rejection Mediated by Low Affinity Memory CD8+ T Cells During Heterologous Immunity. Journal of immunology (Baltimore, Md. : 1950), 197(5), 2009-2015.

Publication 2016

N4 OVA peptide Ready-Set-Go ELISA kit

Cells Elisa Epitope Foot Infection Listeria monocytogenes Mice Peptide Serum Strains

Corresponding Organization : Emory University

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Variable analysis

independent variables

Thy1.1+ OT-I cells (1.0 × 10^4) transferred i.v.
Mice infected with 10^4 CFU Listeria monocytogenes strains expressing OVA APL epitope (LM-OVA APLs) 24 h later
Immunization with 50 µg N4 OVA peptide emulsified in IFA in both hind foot pads 4 weeks post-infection

dependent variables

Secondary effectors generated in 4 week post-infection mice
Serum analyzed using Ready-Set-Go ELISA kit

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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