Primary cortical cultures were lysed in 1 ×RIPA buffer (Pierce biotechnology, Rockford, IL) with complete protease and phosphatase inhibitors (Roche, Indianapolis, IN), lysates were spun at 1000×g for 10 min and supernatants (referred to as RIPA extracts herein) were saved for further analyses. Brain tissues (frontal cortices) were homogenized in TEVP+ 320 mM sucrose to obtain total homogenates and crude synaptic membrane fractions (P2) as described [37 (link),17 (link)]. Samples were resolved on 8% SDS-PAGE. Proteins were transferred onto nitrocellulose membranes (Bio-Rad, Richmond, CA) and incubated with specific primary antibodies (as listed in Supplementary Table 1) overnight at 4 °C. On the second day, membranes were incubated with peroxidase-conjugated secondary antibodies (Pierce, 1:5,000) for 2 h at RT. Immunoreactivity was developed using a Chemiluminescent Substrate kit (Pierce) and visualized by a G:BOX with the GeneSnap software (Syngene, Cambridge, UK). All densitometric bands were quantified using the Genetools program (Syngene).