In Vitro Angiogenesis Assay

Matrigel Matrix 356237 (Corning Inc., Life Sciences, MA) was coated on 15-well μ-angiogenesis slides at 10 μL/well (ibidi GmbH, Planegg, Germany). The coated slides were incubated for 15 minutes at 37°C. Treated HUVECs (10,000 cells/well) were harvested and seeded into the Matrigel-containing wells and incubated for 6 hours at 37°C to allow tube formation. The wells were then imaged for capillary-like structures using an inverted microscope (Life Technologies). Quantification of the tubes was performed by taking 4 images of each chamber, which were then analyzed by ImageJ for in vitro angiogenesis [33 (link)].