Molecular Characterization of Clinical Klebsiella pneumoniae Isolates

K. pneumoniae A1705 and 911021 strains were isolated from the urine samples of patients attending teaching hospitals in Shenyang and Chongqing, respectively. K. pneumoniae 1642 was isolated from a bronchoalveolar lavage fluid of a patient in the 307^th Hospital of the People’s Liberation Army in Beijing. Bacterial species were identified by the VITEK-2 automated system (BioMerieux Inc., Marcy-l’Etoile, France) and 16S rRNA gene sequencing.20 (link) The multilocus sequence typing (MLST) scheme for K. pneumoniae was followed as mentioned on the homepage (

http://bigsdb.pasteur.fr/klebsiella

)3 (link) by utilizing seven housekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB, and tonB).21 (link) PCR screening of ESBLs,22 (link) carbapenem resistance,23 (link) quinolone resistance,24 (link) macrolide resistance25 (link) and common tetracycline resistance genes26 (link) was carried out for each strain. All PCR amplicons were sequenced on an ABI 3730 platform (Applied Biosystems, CA, USA).

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Nazir A., Zhao Y., Li M., Manzoor R., Tahir R.A., Zhang X., Qing H, & Tong Y. (2020). Structural Genomics of repA, repB1-Carrying IncFIB Family pA1705-qnrS, P911021-tetA, and P1642-tetA, Multidrug-Resistant Plasmids from Klebsiella pneumoniae. Infection and Drug Resistance, 13, 1889-1903.

Publication 2020

VITEK-2 automated system ABI 3730 platform

16s rrna Bacterial Bronchoalveolar lavage fluid Carbapenem Gene Housekeeping genes K pneumoniae Klebsiella Macrolide Patient Quinolone Strain Urine

Corresponding Organization :

Other organizations : Ministry of Industry and Information Technology, Beijing Institute of Technology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

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Variable analysis

independent variables

Bacterial strains isolated from different sources (urine, bronchoalveolar lavage fluid)

dependent variables

Antibiotic resistance profiles (ESBL, carbapenem, quinolone, macrolide, tetracycline)

control variables

Bacterial species identification (VITEK-2 automated system and 16S rRNA gene sequencing)
Multilocus sequence typing (MLST) scheme for K. pneumoniae

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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