NTR1 (C47H80O18, molecular weight 933.16, Fig. 1A ), with a purity > 99.0%, was purchased from the Chinese National Institutes for Food and Drug Control (Being, China). NTR1 was dissolved in sterile H2O to make 1 mM stock solution and stored at −20°C.
Human Aβ1-42 oligomers were prepared from commercially available synthetic peptides (American Peptide Company, Inc., Sunnyvale, CA) as described56 (link). Briefly, the lyophilized peptide (1 mg) was resuspended in 250 µL cold 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Sigma) and aliquoted in 3 polypropylene vials. After 2 hours of incubation under the hood, HFIP was removed under gentle vacuum to form a thin film of peptide. Sealed vials were then stored at –20°C. Prior to use, 14.8 µL anhydrous DMSO (Sigma) was added to one sealed vial to obtain a pure monomeric Aβ/DMSO solution that was sonicated for 10 min and then aliquoted by 1.6 µL per vial (5 mM). Oligomeric Aβ42 was obtained by incubating an aliquot of monomeric Aβ/DMSO solution in 100 µL sterile PBS or ACSF (80 µM) at 4°C for 24 hours.
Human Aβ1-42 oligomers were prepared from commercially available synthetic peptides (American Peptide Company, Inc., Sunnyvale, CA) as described56 (link). Briefly, the lyophilized peptide (1 mg) was resuspended in 250 µL cold 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Sigma) and aliquoted in 3 polypropylene vials. After 2 hours of incubation under the hood, HFIP was removed under gentle vacuum to form a thin film of peptide. Sealed vials were then stored at –20°C. Prior to use, 14.8 µL anhydrous DMSO (Sigma) was added to one sealed vial to obtain a pure monomeric Aβ/DMSO solution that was sonicated for 10 min and then aliquoted by 1.6 µL per vial (5 mM). Oligomeric Aβ42 was obtained by incubating an aliquot of monomeric Aβ/DMSO solution in 100 µL sterile PBS or ACSF (80 µM) at 4°C for 24 hours.
