Immunofluorescence staining was performed using leaves from 21-day-old DRE2-HA transgenic plant as previously described [59 (link)]. Nuclei preparations were incubated with a mouse anti-HA monoclonal antibody (Sigma, H3663) overnight at room temperature and then incubated with goat anti-mouse Alexa Fluor 488 secondary antibody (Invitrogen, A-11001) for 2 h at 37°C. DNA was counterstained using DAPI in Prolong Gold (Invitrogen, P36931). Pictures were taken using the Nikon’s modular A1+/A1R+ confocal laser scanning microscope system (National Center for Protein Sciences at Peking University, Beijing).
Free full text: Click here
