Bacterial Strains and Plasmids for Recombinant Protein Expression

Bacterial strains and plasmids used in this study are listed in Table 1. The Lactobacillus salivarius IBB3154 strain used in this study was cultured in MRS-liquid or MRS-agar (solidified with 1.5% agar) medium (Difco Laboratories, Detroit, MI, USA) at 37°C (Kobierecka et al., 2015 (link)). The E. coli strain TG1 was used as a host for the construction of recombinant plasmids. The E. coli strain Rosetta (DE3) pLysS was used to overexpress pUWM1414, pUWM1293 and pUWM1313, and E. coli strain BL21 (DE3) was used to overexpress pUWM1282 (Łaniewski et al., 2014 (link)). C. jejuni and E. coli strains were grown under standard conditions (Łaniewski et al., 2014 (link)) unless otherwise indicated. When needed, media were supplemented with antibiotics at the following concentrations: 30 μg ml^-1 kanamycin and 15 μg ml^-1 chloramphenicol, Campylobacter Selective Supplement (Oxoid) and IPTG (3 mg ml^-1) in DMF (dimethyl-formamide).
The 1,2-Dipalmitoyl-sn-glycero-phosphatidylcholine (DPPC), 1,2-Distearoyl-sn-glycero-phosphoethanolamine-N-[poly(ethyl-ene glycol)2000] (DSPE-PEG 2000) and cholesterol (Chol) were purchased from Northern Lipids, Inc. (Vancouver, BC, Canada). Sephadex G-50 fine was obtained from Sigma–Aldrich Chemie GmbH (Steinheim, Germany). All the other reagents were of analytical grade.