Culturing Cancer Cell Lines and Isolating Lung Cells

Cancer cell lines used in this study were cultured in Dulbecco's modified minimal essential medium (Invitrogen) supplemented with heat-inactivated 10% fetal bovine serum (Invitrogen) and antibiotics at 37°C in a humidified incubator with a mixture of 95% air and 5% CO₂. Mouse CT26^Flag−CAGE cells that stably express CAGE were established by selection in medium containing G418 (400 μg /ml). CT26^Flag−CAGE1 cells and CT26^Flag−CAGE2 cells stably express CAGE. These cells are separate independent clone. CT26 cells were purchased from Korea Cell Line Bank (KCLB 80009). Lung mast cells and lung macrophages were isolated according to standard procedures (17 (link)).