Antibody Characterization Protocol for DNA Damage Response

Antibodies used in this study included: UBR5 (#8755, CST^®, 1:1000); anti-mouse-BrdU (Becton Dickinson, different dilutions); anti-rat-BrdU (ab6326, Abcam; 1:500); PCNA-PC10 (Cancer Research, UK, 1:1000); Vimentin (V6389, Sigma-Aldrich, 1:1000); RNF8 (ab15850, Abcam; 1:2000); RNF168 (#ABE367, Millipore; 1:500); Tubulin (clone B-5-1-2, Sigma-Aldrich); Anti-Flag M2 (F3165, Sigma-Aldrich); 53BP1 (NB100-305, Novusbio, 1:2000); Chk1-PhosphoS345 (#2341 CST^®,1:1000); Chk1 (#2360 CST®,1:1000); Chk2-PhosphoT68 (#2661 CST^®, 1:500); Chk2(#2662 CST®, 1:1000); TRIP12 (ab86220, Abcam; 1:500); Histone H2A(ab18255, Abcam; 1:1000); polη (custom made, raised against the peptide: VQVEQRQNPHLRNKPC, 1:1000); polη-PhosphoS601(Eurogentec,(15 (link))); Histone H2A.X-PhosphoS139 (Upstate), RPA2 (1:1000, Millipore).

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Cipolla L., Bertoletti F., Maffia A., Liang C.C., Lehmann A.R., Cohn M.A, & Sabbioneda S. (2019). UBR5 interacts with the replication fork and protects DNA replication from DNA polymerase η toxicity. Nucleic Acids Research, 47(21), 11268-11283.

Publication 2019

anti-mouse-BrdU anti-rat-BrdU ab6326 Vimentin ab15850 RNF168 Anti-Flag M2 Histone H2A ab18255

53bp1 Antibodies Brdu Cancer Clone Dilutions Histone h2a Histone h2a x Mouse Pcna Peptide Rpa2 Tubulin Vimentin

Corresponding Organization : Istituto di Genetica Molecolare

Other organizations : University of Oxford, University of Sussex

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