Confocal time-lapse imaging of Tribolium embryos injected with GAP43-YFP mRNA was performed as previously described (Benton et al., 2013 (link); Benton, 2018 (link)) at 25–32°C at time intervals from 2 to 10 min between timepoints using 20x, 40x, or 63x objectives. For live imaging of the posterior poles, eggs were propped up vertically (resting against another egg for stability) on a glass-bottomed Petri dish (MatTek) with their posterior against the glass. Live imaging transgenic nuclear GFP (nGFP) (Sarrazin et al., 2012 (link)) or LifeAct-GFP (van Drongelen et al., 2018 (link)) embryos was done at room temperature using the Zeiss AxioImager.Z2 in combination with an Apotome.2 and movable stage (Zen2 Blue).
For imaging Gryllus bimaculatus embryos we used a Zeiss AxioZoom.V16, equipped with a movable stage. Gryllus embryos were placed on 1.5% agarose and were covered with Voltalef H10S oil (Sigma). Imaging was performed at 25–27° C.
For imaging Gryllus bimaculatus embryos we used a Zeiss AxioZoom.V16, equipped with a movable stage. Gryllus embryos were placed on 1.5% agarose and were covered with Voltalef H10S oil (Sigma). Imaging was performed at 25–27
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