The methods applied for anthropometric and biochemical measurements were carried out as previously described [20 (link)]. Briefly, the participants provided their medical history, completed a lifestyle questionnaire, and underwent a health examination. Muscle, body fat, and visceral fat contents were measured via bioelectrical impedance analysis using the InBody Body Composition Analyzer (InBody, Seoul, Korea). Venous blood samples were obtained to measure TG, HDL-C, low density lipoprotein cholesterol (LDL-C), glucose, insulin, glycosylated hemoglobin (HbA1c), aspartate transaminase, alanine transaminase, gamma-glutamyl transferase, and C-reactive protein (CRP) levels before and after the follow-up. The baseline serum ANGPTL6 levels were assessed with an enzyme-linked immunosorbent assay kit (Adipogen Life Science, San Diego, CA, USA) using serum aliquots stored at −80℃ according to manufacturer's instruction. The inter- and intra-assay coefficient of variation of ANGPTL6 measurement were 10.08% and 5.45%, respectively.