CRC cells were seeded in a clinically relevant 1:1 ratio with CCD18co fibroblasts and 5% ECRF24 endothelial cells in 96-well U-bottom low attachment plates (650970, Greiner Bio-One, Frickenhausen, Germany) [31 (link),32 (link)]. The 3Dcc culture media contained a mixture of DMEM-RPMI-EMEM (1:1:1) supplemented with 2.5% Matrigel® (354254, Corning, Bedford, MA, USA). The 3D-CCs were treated with drugs 48 h post-seeding.
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