3D co-culture drug screening protocol
Corresponding Organization : HES-SO University of Applied Sciences and Arts Western Switzerland
Other organizations : University Hospital of Geneva
Variable analysis
- Drug treatment
- Cell viability/proliferation
- Cell line ratio (CRC cells: CCD18co fibroblasts: ECRF24 endothelial cells = 1:1:0.05)
- Culture media (DMEM-RPMI-EMEM (1:1:1) supplemented with 2.5% Matrigel®)
- Culture vessel (96-well U-bottom low attachment plates)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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