Genome Sequencing of Solitarious Locusts

The strain for genome sequencing originated from inbred laboratory strains of solitarious locusts, which were produced from eight generations of sib mating at the Institute of Zoology, CAS, China. DNA for genome sequencing was extracted from the whole body of one female adult, with the exception of its guts. Genomic DNA was isolated using standard molecular biology techniques. Gradient increased insert-size libraries, 170, 200, 500 and 800 bp and 2, 5, 10, 20 and 40 kb were constructed. All libraries were sequenced on Illumina HiSeq 2000. In total, 42 paired-end sequencing libraries were constructed and 82 lanes were sequenced, producing 1,135 Gb of raw data. Further low-quality and duplicated reads filtering resulted in 720 Gb (114 × coverage) data for genome assembly (Supplementary Table S1).