Confocal imaging and time-course imaging were done as previously described (Rafalska-Metcalf et al., 2010 (link); Newhart et al., 2013b (link)). Pearson’s r was calculated using SimplePCI software (Hamamatsu, Middlesex, NJ) by manually selecting the transgene array site with the region of interest (ROI) function. Intensity profiles for merged pictures were also generated using SimplePCI software.
Protocol detail
Quantitative Confocal Imaging of Transgene Arrays
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Function region
Transgene
Corresponding Organization : The Wistar Institute
Other organizations : University of the Sciences, Drexel University
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Variable analysis
independent variables
- Confocal imaging
- Time-course imaging
- Pearson's r (calculated using SimplePCI software)
- Intensity profiles for merged pictures (generated using SimplePCI software)
- Region of interest (ROI) function in SimplePCI software
- No positive or negative controls were explicitly mentioned.
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