Imaging experiments were conducted on an Olympus FluoView 1000 laser scanning confocal microscope and an Olympus IX81-ZDC inverted microscope. Biosensor imaging was performed as previously described8 (link),29 . Simultaneous biosensor imaging and activation of PA-Rac was achieved using a MAG Biosystems FRAP-3D add-on (Photometrics) for galvanometer control of laser position. Detailed materials and methods are included in the supplementary information .
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Simultaneous Biosensor Imaging and PA-Rac Activation
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Biosensor
Confocal laser scanning microscope
Microscope
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Variable analysis
independent variables
- Activation of PA-Rac
- Biosensor imaging
- Experimental conditions described in the supplementary information
- Positive control: Biosensor imaging as previously described in the referenced publication (link provided)
- Negative control: Not explicitly mentioned
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