Cell proliferation after transfection with hsa_circ_0061276 overexpressing plasmids or hsa_circ_0061276 shRNA plasmids (Geneseed) was detected using Cell Counting Kit-8 (CCK-8) (Dojindo, Tokyo, Japan). After culturing for 24 h, 48 h, 72 h, and 96 h, 10 μl/well of CCK-8 reagent was added to the cultures in the dark, and then the cultures were incubated for 3 h. The SpectraMax M5 reader (Molecular Devices, Silicon Valley, CA, USA) was used to detect the plate optical density (OD) at 450 nm.
Forty-eight hours post-transfection with hsa_circ_0061276 overexpressing plasmids or shRNA plasmids (Geneseed), colony formation experiments were performed as previously reported (19 (link)). Forty-eight hours after cell transfection, cells were inoculated into a six-well plate (1,000 cells per well). After culturing cells for 10 days, colonies were first stained with crystal violet (Solarbio, Beijing, China) and then counted using Photoshop software (Adobe, San Jose, CA, USA).
Forty-eight hours post-transfection with hsa_circ_0061276 overexpressing plasmids or shRNA plasmids (Geneseed), colony formation experiments were performed as previously reported (19 (link)). Forty-eight hours after cell transfection, cells were inoculated into a six-well plate (1,000 cells per well). After culturing cells for 10 days, colonies were first stained with crystal violet (Solarbio, Beijing, China) and then counted using Photoshop software (Adobe, San Jose, CA, USA).
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