Antifungal Screening of Candida Species

Three American Type Culture Collection (ATCC) yeast species, including Candida albicans ATCC 36082 (fluconazole-resistant), Candida glabrata ATCC 2001 (fluconazole susceptible), Candida parapsilosis ATCC 22019 (fluconazole-resistant) were obtained from Oxoid, UK and Candida albicans CL1 (fluconazole-resistant) was recovered from scalp infection in this study [62 (link),63 (link),64 (link)]. Pure cultures were grown onto sabouraud dextrose agar (SDA, Merck, Germany) at 28 °C for 48 h, as described by Pfaller et al. [65 (link)]. Standard solutions of 1500 µg/mL of fluconazole (FLZ) (Pfizer, Manhattan, NY, USA) and synthetic compounds were prepared in DMSO (Sigma Aldrich, Germany).

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Khan F.A., Yaqoob S., Ali S., Tanveer N., Wang Y., Ashraf S., Hasan K.A., Khalifa S.A., Shou Q., Ul-Haq Z., Jiang Z.H, & El-Seedi H.R. (2022). Designing Functionally Substituted Pyridine-Carbohydrazides for Potent Antibacterial and Devouring Antifungal Effect on Multidrug Resistant (MDR) Strains. Molecules, 28(1), 212.

Publication 2022

sabouraud dextrose agar (SDA, fluconazole (FLZ) DMSO

Agar Candida albicans Candida glabrata Candida parapsilosis Dextrose Dmso Fluconazole Infection Scalp Yeast

Corresponding Organization : Uppsala University

Other organizations : Stockholm University, Zhejiang Chinese Medical University, Lakehead University

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Variable analysis

independent variables

Candida albicans ATCC 36082 (fluconazole-resistant)
Candida glabrata ATCC 2001 (fluconazole susceptible)
Candida parapsilosis ATCC 22019 (fluconazole-resistant)
Candida albicans CL1 (fluconazole-resistant)

dependent variables

Not explicitly mentioned

control variables

Growth conditions: Sabouraud dextrose agar (SDA) at 28 °C for 48 h
Standard solutions: 1500 µg/mL of fluconazole (FLZ) in DMSO

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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