Apoptotic Cell Quantification in Tissue Sections

Immunohistochemistry analyses were performed as described previously ^{17 (link)}. For apoptotic cells analysis, paraffin-embedded tissue sections were stained with anti-Perilipin (Abcam, Cat. No. ab-61682) and cleaved caspase-3 (Cell Signaling Technology, Cat No. 9664) antibodies. Images were captured using a NanoZoomer slide scanner system with NanoZoomer Digital Pathology software (Hamamatsu) or confocal fluorescence microscopy. Microscopic images were analyzed using ImageJ software.

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Seo J.B., Riopel M., Cabrales P., Huh J.Y., Bandyopadhyay G.K., Andreyev A.Y., Murphy A.N., Beeman S.C., Smith G.I., Klein S., Lee Y.S, & Olefsky J.M. (2018). Knockdown of Ant2 Reduces Adipocyte Hypoxia And Improves Insulin Resistance in Obesity. Nature metabolism, 1(1), 86-97.

Publication 2018

anti-Perilipin cleaved caspase-3 NanoZoomer slide scanner system NanoZoomer Digital Pathology software

Antibodies Apoptotic Caspase 3 Cells Confocal microscopy Fluorescence Fluorescence microscopy Immunohistochemistry Microscopic Paraffin Perilipin Scanner Tissue

Corresponding Organization :

Other organizations : University of California, San Diego, Washington University in St. Louis

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Variable analysis

independent variables

Anti-Perilipin (Abcam, Cat. No. ab-61682) antibody
Cleaved caspase-3 (Cell Signaling Technology, Cat No. 9664) antibody

dependent variables

Apoptotic cells

control variables

Paraffin-embedded tissue sections

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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