Quantification of MeO-PCBs in Biological Samples

Because of the high chromatographic resolution, OH-PCBs are typically extracted from biological or environmental matrices, derivatized to MeO-PCBs, and analyzed by GC-MS/MS.^{4 (link), 14 (link), 15 (link), 58 (link)} We measured the RRTs and MS/MS profiles [expressed as the relative intensities of five multiple reaction monitering (MRM) transitions] of two MeO-PCB standard solutions (Solution 1 containing 72 MeO-PCBs and Solution 2 containing 52 MeO-PCBs; see Supporting Information for additional information) using an Agilent 7890B gas chromatograph equipped with an SPB-Octyl capillary column (30 m length, 250 μm inner diameter, 0.25 μm film thickness; Supelco, Bellefonte, PA, USA), an Agilent 7000D Triple Quad and an Agilent 7693 sampler. These data were used as dependent variables for the model development. For additional details, see the Supporting Information.

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Zhang C.Y., Li X., Keil Stietz K.P., Sethi S., Yang W., Marek R.F., Ding X., Lein P.J., Hornbuckle K.C, & Lehmler H.J. (2022). Machine Learning-Assisted Identification and Quantification of Hydroxylated Metabolites of Polychlorinated Biphenyls in Animal Samples. Environmental Science & Technology, 56(18), 13169-13178.

Publication 2022

Agilent 7890B gas chromatograph SPB-Octyl capillary column Agilent 7000D Triple Quad

Biological Capillary Chromatographic Gas chromatograph Gc ms Ms ms Pcbs Rrts

Corresponding Organization : University of Iowa

Other organizations : University of California, Davis, University of Arizona

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Variable analysis

independent variables

Measurements of the RRTs and MS/MS profiles [expressed as the relative intensities of five multiple reaction monitering (MRM) transitions] of two MeO-PCB standard solutions (Solution 1 containing 72 MeO-PCBs and Solution 2 containing 52 MeO-PCBs)

dependent variables

RRTs and MS/MS profiles [expressed as the relative intensities of five multiple reaction monitering (MRM) transitions] of two MeO-PCB standard solutions

control variables

Agilent 7890B gas chromatograph equipped with an SPB-Octyl capillary column (30 m length, 250 μm inner diameter, 0.25 μm film thickness; Supelco, Bellefonte, PA, USA)
Agilent 7000D Triple Quad
Agilent 7693 sampler

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