Comprehensive Lung Cancer Transcriptome Analysis

A total of 66 samples were used for microarray analysis, including paired adjacent normal-tumor samples from 27 patients underwent surgery for lung cancer at the Taipei Veterans General Hospital, two tissue mixtures from the Taichung Veterans General Hospital (one was adjacent normal lung mixtures and the other was lung adenocarcinoma mixtures), two commercial human normal lung tissues (Clontech (Catalog No. 636524) and Stratagene (Catalog No. 735020)), one immortalized, nontumorigenic human bronchial epithelial cell line (NL-20 (ATCC^®No. CRL-2503™)) and 7 lung cancer cell lines (A-549 (ATCC^®No. CCL-185™), NCI-H1299 (ATCC^®No. CRL-5803™), NCI-H661 (ATCC^®No. HTB-183™), CL_1-0[33 (link)], CL_1-1[33 (link)], CL_1–5[33 (link)], and CL_1–5-F4 [34 (link)]).

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Su L.J., Chang C.W., Wu Y.C., Chen K.C., Lin C.J., Liang S.C., Lin C.H., Whang-Peng J., Hsu S.L., Chen C.H, & Huang C.Y. (2007). Selection of DDX5 as a novel internal control for Q-RT-PCR from microarray data using a block bootstrap re-sampling scheme. BMC Genomics, 8, 140.

Publication 2007

Bronchial Cancer Cell lines Human Lung Lung adenocarcinoma Lung cancer Microarray analysis Normal tissues Patients Surgery Surgery lung Tumor

Corresponding Organization :

Other organizations : National Health Research Institutes, Academia Sinica, Taipei Veterans General Hospital, National Chung Cheng University, National Yang Ming Chiao Tung University, Taichung Veterans General Hospital, National Taiwan University, Institute of Statistical Science, Academia Sinica

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Protocol cited in 29 other protocols

Variable analysis

independent variables

Lung cancer samples from 27 patients
Tissue mixtures from Taichung Veterans General Hospital (adjacent normal lung mixtures and lung adenocarcinoma mixtures)
Commercial human normal lung tissues (Clontech and Stratagene)
Immortalized, nontumorigenic human bronchial epithelial cell line (NL-20)
Lung cancer cell lines (A-549, NCI-H1299, NCI-H661, CL1-0, CL1-1, CL1-5, CL1-5-F4)

dependent variables

Unspecified

control variables

Unspecified

positive controls

Commercial human normal lung tissues (Clontech and Stratagene)
Immortalized, nontumorigenic human bronchial epithelial cell line (NL-20)

negative controls

Lung cancer cell lines (A-549, NCI-H1299, NCI-H661, CL1-0, CL1-1, CL1-5, CL1-5-F4)

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