The genome-wide expression data of murine podocytes, mesangial, and endothelial cells were obtained from http://www.gudmap.org/. The identifications of data sets that have been utilized in our study are listed in Supplemental Table 5. The detailed protocol of data generation is described in a recently published paper by Brunskill et al. (2011) (link) and can also be found in our Supplemental Methods. We preprocessed and normalized data as described in Microdissected Human Kidney Data. By comparing the expression level in podocytes versus the other two major cell types in glomeruli, we define a gene to be podocyte-specific if its expression in podocytes is 4.76-fold over mesangial cells and 4.65-fold over glomerular capillary endothelial cells. The cut-off values represent three standard deviations of the average difference between podocytes and mesangial/endothelial cell transcripts, respectively. By use of HomoloGene from NCBI Entrez (Maglott et al. 2011 (link)), 102 murine genes could be mapped to their Homo sapiens ortholog (Supplemental Table 6).