Ophthalmological Assessment in Glaucoma Model

At the beginning of the study, all animals (n = 24) underwent a complete ophthalmological examination. The neuroophthalmological examination consisted in the evaluation of the palpebral, corneal, pupillary (direct and indirect), and dazzle reflexes, as well as visual acuity through a maze test response. The assessment of the anterior segment was done with a portable Biomicroscope (Hawk Eye^®, Dioptrix, Toulouse, France), and the posterior segment was assessed with a PanOptic^® Ophthalmoscope (WelchAllyn, Hillrom, NY, USA). The IOP measurement was performed with a rebound tonometer (Tonolab^®, Icare, Finland). These procedures were repeated immediately after glaucoma induction, and then after 1 h, 3 h, and 1, 2, 3, 5, and 7 days in all groups, and at 14 and 21 days in cases where applied.