Lysosomal pH Analysis in Astrocytes

For lysosomal pH analysis, the ratiometric dye LysoSensor Yellow/Blue (Invitrogen) was used to label 10,000 astrocytes per well in 96-well black wall black bottom plates (Greiner) as previously described.^{6 (link)}

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Sanyal A., DeAndrade M.P., Novis H.S., Lin S., Chang J., Lengacher N., Tomlinson J.J., Tansey M.G, & LaVoie M.J. (2020). Lysosome and Inflammatory Defects in GBA1-Mutant Astrocytes Are Normalized by LRRK2 Inhibition. Movement disorders : official journal of the Movement Disorder Society, 35(5), 760-773.

Publication 2020

LysoSensor Yellow/Blue

Astrocytes Lysosensor yellow blue Lysosomal

Corresponding Organization : Harvard University

Other organizations : Emory University, Ottawa Hospital, University of Ottawa, Florida College, University of Florida

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Variable analysis

independent variables

LysoSensor Yellow/Blue (Invitrogen) dye

dependent variables

Lysosomal pH

control variables

Number of astrocytes per well (10,000)
Plate type (96-well black wall black bottom plates, Greiner)

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