Protocol detail

Blastocystis Extraction from Fecal Samples

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Total genomic DNA was extracted directly from approximately 250 mg of animal and human fecal samples using the QIAamp DNA Stool Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s recommended procedures. DNA was eluted in 200 μl of elution buffer and stored at -20 °C at the Department of Microbiology of the AZM Center of Tripoli. DNA samples were then transported to the Pasteur Institute in Lille (France) for molecular screening and subtyping of Blastocystis sp.

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Greige S., El Safadi D., Bécu N., Gantois N., Pereira B., Chabé M., Benamrouz-Vanneste S., Certad G., El Hage R., Chemaly M., Hamze M, & Viscogliosi E. (2018). Prevalence and subtype distribution of Blastocystis sp. isolates from poultry in Lebanon and evidence of zoonotic potential. Parasites & Vectors, 11, 389.

Publication 2018

QIAamp DNA Stool Mini Kit

Animal Blastocystis Buffer Genomic Human Stool

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Protocol cited in 4 other protocols

Variable analysis

independent variables

DNA extraction method (QIAamp DNA Stool Mini Kit)
Fecal sample source (animal and human)

dependent variables

Presence/absence of Blastocystis sp.

control variables

Sample size (approximately 250 mg of fecal samples)
DNA elution volume (200 μl of elution buffer)
DNA storage temperature (-20 °C)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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