Evaluation of IFN-γ and TGF-β in Tumor-Bearing Mice

As described above [19 (link)], splenic cells from differently treated and non-treated tumor-bearing mice were incubated with MMC inactivated CT26 (alive but not proliferative) at a R:S ratio of 10:1. The supernatant of the mixed lymphocyte and tumor cell culture (MLTC) was collected on day 3, and the concentrations of IFN-γ were determined using the mouse IFN-γ ELISA kit (Dakewe Biotech, Shenzhen, China). Serum from treated and control mice were collected and detected via the TGF-β ELISA kit (NeoBioscience Ltd., Beijing, China).

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Wu Y., Deng Z., Wang H., Ma W., Zhou C, & Zhang S. (2016). Repeated cycles of 5-fluorouracil chemotherapy impaired anti-tumor functions of cytotoxic T cells in a CT26 tumor-bearing mouse model. BMC Immunology, 17, 29.

Publication 2016

mouse IFN-γ ELISA kit

Cell Elisa Ifn γ Mice Mixed lymphocyte culture Mouse ifn γ Serum Splenic Tgf β Tumor

Corresponding Organization :

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Treatment of tumor-bearing mice

dependent variables

Concentration of IFN-γ in the supernatant of the mixed lymphocyte and tumor cell culture (MLTC)
Concentration of TGF-β in the serum of treated and control mice

control variables

R:S ratio of 10:1 in the MLTC
Incubation of splenic cells from differently treated and non-treated tumor-bearing mice with MMC inactivated CT26 (alive but not proliferative)

controls

Positive control: Serum from treated mice
Negative control: Serum from control mice

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