Novel Flavonoid Derivative GL-V9 Evaluation

GL-V9 (C₂₄H₂₇NO₅,(5-hydroxy-8-methoxy-2-phenyl-7-(4-(pyrrolidin-1-yl) butoxy)4H-chromen-4-one), MW 409.47, purity 99%) is a new flavonoid derivative and synthesize by Prof. Zhiyu Li in our lab [25] (link). GL-V9 was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich, St. Louis, MO, USA), made into the parent solution with the concentration of 0.1 M, and then stored at − 80 °C. The final concentration is 2.5, 5, 10, 15 and 20 μM, diluted in Dulbecco's modified Eagle medium (DMEM; GIBCO, Carlsbad, CA, USA).
Paclitaxel injection was purchased from Haikou Pharmaceutical Factory. Dorsomorphin Dihydrochloride (Compound C, C₂₄H₂₇ClN₅O, MW 472.41 and purity 99.73%) was purchased from MedChem Express and dissolved in DMSO to 5 mM. N-acetyl-L-cysteine, which was from Beyotime Biotechnology, was dissolved in water to 0.5 M and was diluted with DMEM to its final concentration. Etomoxir (C₁₅H₁₈ClNaO_4, MW 320.74 and purity 98.63%) was from MedChem Express and dissolved in water to 10 mM. Dehydroepiandrosterone (DHEA, C₁₉H₂₈O₂, MW 288.42) was from Solarbio Life Sciences and dissolved in DMSO to 1 M.