Multimodal Imaging of Liver Pathologies

Masson’s Trichrome and immunohistochemistry was done as described before^{21 (link),22 (link)}. For immunofluorescence staining, liver sections were blocked (1% bovine serum albumin in 0.5% PBS-TritonX) and incubated in primary antibodies: F4/80 (AbD Serotec), GFP (Rockland), and BrdU (Cell Signaling). The primary antibodies were detected using AF488 (Vector) and AF647 (Vector). Proliferation in hepatic cells was measured using BrdU uptake. Mice received an IP injection of BrdU 24 h prior to death (50 mg/kg of animal weight).

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Amini-Nik S., Sadri A.R., Diao L., Belo C, & Jeschke M.G. (2018). Accumulation of myeloid lineage cells is mapping out liver fibrosis post injury: a targetable lesion using Ketanserin. Experimental & Molecular Medicine, 50(7), 1-13.

Publication 2018

F4/80 BrdU

Af647 Animal Antibodies Bovine serum albumin Brdu Cells proliferation Immunofluorescence Immunohistochemistry Ip injection Liver Mice Vector

Corresponding Organization : Sunnybrook Hospital

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Variable analysis

independent variables

Masson's Trichrome and immunohistochemistry protocol
Immunofluorescence staining protocol (blocking, primary antibodies, and secondary antibodies used)

dependent variables

Proliferation in hepatic cells measured using BrdU uptake

control variables

Mice received an IP injection of BrdU 24 h prior to death (50 mg/kg of animal weight)

controls

None specified
None specified

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