Generation of Infectious HIV-1 Molecular Clones

The full-length genomes for T/F viruses were inferred as described in previous studies14 (link)25 (link). Infectious molecular clones (IMCs) were chemically synthesized and constructed for different T/F viruses from three subjects (CH0200, CH0228 and CH0078) as previously described2 (link)14 (link). Among them, CH0200a, CH0228a and CH0228b were reported in a previous study16 (link). The viral stocks were generated by transfecting the IMCs into 293T cells as described previously25 (link). The p24 concentrations of viral stocks were determined with the p24 ELISA kit (PerkinElmer, Waltham, MA) and TCID₅₀ of viral stocks was determined on TZM-bl cells.

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Song H., Hora B., Giorgi E.E., Kumar A., Cai F., Bhattacharya T., Perelson A.S, & Gao F. (2016). Transmission of Multiple HIV-1 Subtype C Transmitted/founder Viruses into the Same Recipients Was not Determined by Modest Phenotypic Differences. Scientific Reports, 6, 38130.

Publication 2016

p24 ELISA kit

293t cells Cells Clones Elisa Infectious Viruses Viruses genomes

Corresponding Organization :

Other organizations : Duke University Hospital, Duke Medical Center, Los Alamos National Laboratory, Jilin University

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Variable analysis

independent variables

Infectious molecular clones (IMCs) of different T/F viruses from three subjects (CH0200, CH0228 and CH0078)

dependent variables

P24 concentrations of viral stocks
TCID50 of viral stocks

control variables

Transfection of IMCs into 293T cells

controls

Positive control: Not specified
Negative control: Not specified

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