Total RNA from mouse cerebellum was prepared using EuroGOLD Trifast (Euroclone, Milano, Italy). One microgram of total RNA was reverse-transcribed, as previously described [18 (link)]. Total cDNA (1 μL) was used to perform qPCR using the specific primers listed in Additional file 1 . qPCR was performed using the iQ SYBR Green Supermix (Bio-Rad) and a C1000 Thermal Cycler CFX96 Real Time System (Bio-Rad). Expression of the gene of interest was normalized to the Gapdh housekeeping gene. Data were analysed using the ΔΔCt method. All primers are listed in Additional file 1 .
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