The embryonic cell line from A. sculptum IBU/ASE-16 [24 ] was cultured in commercial Leibovitz L-15 culture medium containing gentamicin sulfate and amphotericin (Vitrocell Embriolife, Campinas, SP, Brazil) and supplemented with 0.01% L-glutamine (Sigma‒Aldrich, St. Louis, MO, USA), as previously described [17 (link)].
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