Culturing Thymic Carcinoma Cell Lines

The human thymic carcinoma cell line Ty-82 was obtained from the Japanese Collection of Research Bioresources (JCRB) [46 (link)]. Ty-82 cells were authenticated and certified as uninfected by the JCRB at the time of purchase. We used the cells within 4 months after purchase. Ty-82 cells were cultured in a flask designed only for culturing nonadherent cell lines (Corning, NY, USA). MP57 is a human thymic carcinoma cell line kindly provided by Dr. Giuseppe Giaccone [47 (link)]. Both cells were cultured in RPMI-1640 supplemented with penicillin/streptomycin and 10% fetal bovine serum (FBS) in an atmosphere containing 5% CO₂ in a humidified incubator at 37° C. To induce hypoxia, cells were cultured in the presence of 1% O₂ using a BIONIX-1 hypoxic culture kit (Sugiyama-Gen, Tokyo, Japan).