The BILBO1 ORF was cloned into the pcDNA3 vector as described in [44 (link)], from which Edge-Mut and Centre-Mut were generated using the QuickChange II site-directed mutagenesis kit (Agilent). The FPC4, FPC4-B1BD, FPC4-ΔB1BD, and shuffled FPC4-1-217 ORFs were cloned into pcDNA3.1 CT-GFP TOPO (Invitrogen) in frame with a C-terminal GFP tag. Shuffled FPC4-1-217 DNA sequence was synthesised by Eurofins (Germany).
Free full text: Click here