Cloning and Mutagenesis of BILBO1 ORF
Corresponding Organization : Medical University of Vienna
Protocol cited in 2 other protocols
Variable analysis
- Generation of Edge-Mut and Centre-Mut from the BILBO1 ORF cloned into the pcDNA3 vector
- Cloning of the FPC4, FPC4-B1BD, FPC4-ΔB1BD, and shuffled FPC4-1-217 ORFs into pcDNA3.1 CT-GFP TOPO vector
- Outcome of the experiments involving the generated mutants and constructs
- The pcDNA3 and pcDNA3.1 CT-GFP TOPO vectors used for cloning
- The QuickChange II site-directed mutagenesis kit used to generate the mutants
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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