Phospholipid Profiling by HPLC

The qualitative and quantitative determination of individual phospholipid fractions—phosphatidylcholine (PC), phosphatidylethanolamine (PEA), phosphatidylserine (PS), phosphatidylinositol (PI), and lysophosphatidylcholine (LPC)—was performed by high-performance liquid chromatography (HPLC) and previously described in [11 (link)]. Phospholipid standards (Sigma-Aldrich, USA) were used for identifying and quantifying the phospholipid compounds in the sample. We identified six phospholipids (as total certain fractions without focusing on molecular species): PS, PE, PI, PC, LPC, and SM.

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Voronin V.P., Nemova N.N., Ruokolainen T.R., Artemenkov D.V., Rolskii A.Y., Orlov A.M, & Murzina S.A. (2021). Into the Deep: New Data on the Lipid and Fatty Acid Profile of Redfish Sebastes mentella Inhabiting Different Depths in the Irminger Sea. Biomolecules, 11(5), 704.

Publication 2021

Phospholipid standards

Fractions phospholipid High performance liquid chromatography Lysophosphatidylcholine Phosphatidylcholine Phosphatidylethanolamine Phosphatidylinositol Phosphatidylserine Phospholipids

Corresponding Organization : Institute of Biology of Karelian Research Centre

Other organizations : All-Russian Research Institute Fisheries and Oceanography, Knipovich Polar Research Institute of Marine Fisheries and Oceanography, Institute of Oceanology. PP Shirshov Russian Academy of Sciences, Severtsov Institute of Ecology and Evolution, National Research Tomsk State University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Phospholipid fractions: phosphatidylcholine (PC), phosphatidylethanolamine (PEA), phosphatidylserine (PS), phosphatidylinositol (PI), and lysophosphatidylcholine (LPC)

control variables

Phospholipid standards (Sigma-Aldrich, USA) were used for identifying and quantifying the phospholipid compounds in the sample.

controls

Positive control: Phospholipid standards (Sigma-Aldrich, USA)
Negative control: Not explicitly mentioned

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