Arabidopsis Salt Stress Response

All Arabidopsis thaliana lines used in this study are in Columbia-0 (Col.0) background. The IDD4 transgenic lines used are published (Völz et al., 2019b (link)). The MAPK mutant line used is mpk6–2 (SALK_073907). Prior to every experiment, seeds were surface sterilized and stratified at 4°C for 2d. The stratified seeds were plated on the ½ strength Murashige and Skoog (MS) medium with 1% agar and plants were grown in plant growth chambers (Percival Scientific) under 16h light: 8h dark condition at 22°C. Unless stated, the plants were grown for 5d on ½ MS and then to apply salt-stress, were transferred onto fresh with ½ MS ± 100mM NaCl (Sigma) and grown for another 16d.